This book is a tool and resource for scientists engaged in, or preparing for, the selection and optimization process. The authors describe how properties affect in vivo pharmacological activity and impact in vitro assays.
Individual drug-like properties are discussed from a practical point of view, such as solubility, permeability and metabolic stability, with regard to fundamental understanding, applications of property data in drug discovery and examples of structural modifications that have achieved improved property performance.
The authors also review various methods for the screening high throughput , diagnosis medium throughput and in-depth low throughput analysis of drug properties. Expert laboratorians update the classic bioconjugation methods and introduce valuable new techniques that go beyond pure biomedical applications to include elements from advanced organic synthesis, molecular biology, and materials science.
These readily reproducible methods cover the preparation of protein conjugates using covalent and noncovalent conjugation, the synthesis of nucleic acid conjugates using a variety of labeling techniques, and approaches to semisynthetic conjugates of proteins.
Additional chapters address the biofunctionalization of inorganic surfaces, including the on-chip synthesis of peptide nucleic acids to generate microarrays for the high-throughput analysis of RNA and DNA, gold nanaoparticles, and carbon nanotube probes for atomic force microscopy. These readily reproducible methods cover the preparation of biomolecular conjugates using a variety of labeling techniques and semisynthetic approaches.
All the protocols follow the successful Methods in Molecular BiologyTM series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
The book deals with the synthesis and characterization of hydrogels specifically used as drug delivery systems. Each chapter includes the most recent updates about the different starting materials employed and the improvement of their physicochemical and biological properties to synthetize high performing carriers for specific uses. Providing an updated summary of the application of different types of sensors for the analysis of food safety and quality, this book discusses the core principles, current research status, challenges and successful examples for each technology.
In addition, the prospective and future trends for each topic are covered in each chapter. The editor and contributors are all experts in designing and constructing different types of sensors in food analysis, mainly focusing on the determination of food safety and quality.
Sensors, as a new generation of detection technique, have many advantages and the application of sensors in food analysis will continue to grow in the next decades.
However, until now, there has been no book providing the detailed characterization and summary of sensors in food safety and quality analysis that this book provides. New edition of biochemistry textbook which introduces principles and techniques used in undergraduate practical classes.
It addresses the purpose, science, and application of all physical science instrumentation and analysis methods used in current research labs. The book first presents the historical background, concepts, and motivation for using a physical science toolbox to understand biology.
It then familiarizes students from the physical sciences with essential biological knowledge. The author describes the science and application of key tools used in imaging, detection, general quantitation, and biomolecular interaction studies, which span multiple length and time scales of biological processes both in the test tube and in the living organism.
Moving on to theoretical biophysics tools, the book presents computational and analytical mathematical methods for tackling challenging biological questions. It concludes with a discussion of the future of this exciting field. Future innovators will need to be trained in multidisciplinary science to be successful in industry, academia, and government support agencies.
Addressing this challenge, this textbook educates future leaders on the development and application of novel physical science approaches to solve complex problems linked to biological questions. A comprehensive resource on thiol-x chemistries for postgraduates, academics and industrial practitioners interested in polymer and materials applications from leading experts in the field.
It also describes dozens of reactions, with details on hundreds of commercially available reagents and the use of these reagents for modifying or crosslinking peptides and proteins, sugars and polysaccharides, nucleic acids and oligonucleotides, lipids, and synthetic polymers.
Offers a one-stop source for proven methods and protocols for synthesizing bioconjugates in the lab Provides step-by-step presentation makes the book an ideal source for researchers who are less familiar with the synthesis of bioconjugates Features full color illustrations Includes a more extensive introduction into the vast field of bioconjugation and one of the most thorough overviews of immobilization chemistry ever presented. Author : Yukihiro Ozaki Publisher: Academic Press ISBN: Category: Science Page: View: Read Now » Vibrational Spectroscopy in Protein Research offers a thorough discussion of vibrational spectroscopy in protein research, providing researchers with clear, practical guidance on methods employed, areas of application, and modes of analysis.
With chapter contributions from international leaders in the field, the book addresses basic principles of vibrational spectroscopy in protein research, instrumentation and technologies available, sampling methods, quantitative analysis, origin of group frequencies, and qualitative interpretation.
In addition to discussing vibrational spectroscopy for the analysis of purified proteins, chapter authors also examine its use in studying complex protein systems, including protein aggregates, fibrous proteins, membrane proteins and protein assemblies.
Emphasis throughout the book is placed on applications in human tissue, cell development, and disease analysis, with chapters dedicated to studies of molecular changes that occur during disease progression, as well as identifying changes in tissues and cells in disease studies.
Provides thorough guidance in implementing cutting-edge vibrational spectroscopic methods from international leaders in the field Emphasizes in vivo, in situ and non-invasive analysis of proteins in biomedical and life science research more broadly Contains chapters that address vibrational spectroscopy for the study of simple purified proteins and protein aggregates, fibrous proteins, membrane proteins and protein assemblies.
The primary focus is on the extraordinary advantages of specificity and sensitivity obtained by integrating DNA nanostructures in bioanalytical devices.
DNA Nanotechnology for Bioanalysis provides a concise and rigorous description for the fabrication of various types of functional nanostructures by optimized software-aided high-yield synthesis. Following this is the explanation of methods to decorate these nanostructures with molecules such as proteins, metal nanoparticles or bioorganic moieties covalently bonded onto DNA via self-assemblage processes.
Also provided is a concise review on non-canonical DNA structures such as G-quadruplexes and their targeting by small molecules for applications in pharmacology.
Finally, it describes the exciting applications of DNA nanostructures in life sciences and nanomedicine, including ultraspecific molecular delivery, control of cell behavior, analysis of cell lysate and DNA-based nano-tools for super-resolution sub-cellular imaging.
Author : C. It is an umbrella term which encompasses the various modes of spontaneous organization of micrometer-and submicrometer-sized particles into ordered structures of various degrees of complexity, yet it often relies on remarkably simple interactions and mechanisms. Self-assembly is one of the key principles used by nature to construct living matter, where it frequently takes place in a hierarchical fashion.
The primary aim of the school was to cover the most exciting modern aspects of self-assembly in soft condensed matter physics, and to enable Ph. Subjects covered include: colloids with directional bonding; pathways of self-organization; self-assembly hydrodynamics; polymer structure and dynamics; liquid-crystal colloid dispersions; and self-organizing nanosystems. The proceedings also include two reprints from Reviews of Modern Physics, and will be of interest to both students and experts in the field.
Actual examples of utilization include drug development issues, translation to the clinic, market prospects, and industrial commercialization aspects. The applications described are taken from cancer treatment as well as other major therapeutic areas, such as infectious diseases and dermatology. An in-depth discussion on safety, regulatory, and societal aspects rounds off the book.
Written by a top team of editors and authors composed of the leading experts in Europe and the USA who have pioneered the field of nanopharmacy! Author : Toshikazu Hirao Publisher: Elsevier ISBN: Category: Science Page: View: Read Now » Advances in Bioorganometallic Chemistry examines the synthesis, structure and reactivity of bioorganometallics, their pharmaceutical applications, hydrogenase, vitamin Blike systems, and metalloproteins.
It is written by the top researchers in the field and compiled by editors Toshikazu Hirao and Toshiyuki Moriuchi. Developments in this new field of bioorganometallic chemistry, a hybrid between biology and organometallic chemistry, happen very quickly and this comprehensive reference offers the latest research and findings in the field.
The book features a discussion of the synthesis, structure, and reactivity of bioorganometallics, and an examination of hydrogenase-like systems, which were designed to demonstrate catalytic activities and functional properties.
Advances in Bioorganometallic Chemistry also includes a discussion of bioorganometallics as they relate to medicinal chemistry, specifically applications of metalloproteins, metalloenzymes, and applications in bioimaging.
The book concludes with coverage of vitamin Blike systems, including the latest developments in derivatives designed to perform bio-inspired catalytic reactions. This work is a valuable resource for chemists working in organometallic chemistry and biology, including biochemists, bioorganic chemists, bioinorganic chemists, as well as pharmaceutical scientists, medicinal chemists, and students studying in these areas.
Representative authors: R. Fish, T. Moriuchi, T. Hirao, H. Kraatz, H. Takaya, T. Curran, G. Rosenberg, J. Lynam, C. Hartinger, U. Schatzschneider, G. However, a new generation of nanolithographic and patterning techniques has made it possible to develop complex patterns at the nanoscale.
Non-conventional lithography and patterning summarises this new range of techniques and their industrial applications. A number of chapters look at ways of forming and modifying surfaces for patterning. These are complemented by chapters on particular patterning techniques such as soft lithography, ion beam patterning, the use of nanostencils, photolithography and inkjet printing. The book also discusses prototyping and the manufacture of particular devices.
With its distinguished international team of contributors, Non-conventional lithography and patterning is a standard reference for both those researching and using advanced polymers in such areas as microelectronics and biomedical devices. Looks at alternative approaches used to develop complex patterns at the nanoscale Concentrates on state of the art nanolithographic methods Written by a distinguished international team of contributors.
The understanding of the surface characteristics nano-particles is growing significantly with the advent of new analytical techniques. Polymer chemistry is contributing to the development of many new versatile polymers which have abilities to accommodate many different, very reactive chemical groups, and can be used as a diagnostic tool, for better targeting, for more effective therapeutic results as well as for reducing the toxic and side effects of the drugs.
Surface Modification of Nanoparticles for Targeted Drug Delivery is a collection essential information with various applications of surface modification of nanoparticles and their disease specific applications for therapeutic purposes. Written and presented by leading scientists and engineers in their respective fields, the authors offer a clear and detailed understanding of how to carry out nanoparticle functionalization with biomolecules including enzymes , nanoparticle analysis and characterization, in vitro evaluation of nanoparticles using different cell lines and in vitro evaluation of nanoparticles as therapeutics and imaging agents.
Popular Books. Updated chapters describe either the most popular methods or those processes that have evolved the most since the past edition. Additionally, a large portion of the volume is devoted to clinical cytometry. Particular attention is paid to applications of cytometry in oncology, the most rapidly growing area. Skip to content. Bioconjugate Techniques. Author : Greg T.
Bioconjugate Techniques Book Review:. Bioconjugation Book Review:. Chemistry of Bioconjugates. Chemistry of Bioconjugates Book Review:.
Bioconjugation Protocols. Author : Sonny S. Bioconjugation Protocols Book Review:. Author : Christof M. Functional Hydrogels in Drug Delivery. Separation Techniques in Chemistry and Biochemistry. Author : Roy A. Author : Mark C. Biophysics Book Review:. Author : Andrew B. Lowe,Christopher N. Protein Chromatography.
Protein Chromatography Book Review:. Principles and Techniques of Practical Biochemistry. Radiopharmaceutical Chemistry.
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